The creation of transgenic plants often involves the use of DNA sequences from bacteria and other non-plant organisms - in particular as vectors to introduce the desired genes. However, some people are concerned about the use of DNA from such distantly related sources, and regulators require separate rules to be complied with for transgenic plants compared to those derived by selective breeding. Could using plant-derived sequences help address those fears and reduce the regulatory burden for crop biotechnologists?
Tony Conner and his colleagues from Plant and Food Research, New Zealand consider the potential for intragenic vectors in a paper in CAB Reviews. This involves finding DNA fragments within a plant species that are very similar to those from foreign DNA that have been used as vectors for many years. Various techniques have been developed, some involving small amounts of foreign DNA, but fully intragenic plants have no foreign DNA and are entirely composed from plant-derived sequences.